Investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells: <i>in vitro</i> and preliminary <i>in vivo</i> evaluations

Aim To investigate the vascularization capacity of a decellularized dental pulp matrix (DDP) bovine origin seeded with human stem cells (hDPSCs) in vitro and to present preliminary vivo findings. Methodology Bovine pulps were then analysed using histological staining DNA quantification. The resultant DDPs characterized immunohistochemical for retention vascular endothelial growth factor A (VEGF-A) fibroblast 2 (FGF-2). Furthermore, recellularized hDPSCs histologically. expression markers involved angiogenesis by colonizing was assessed vitro. study conducted which hDPSCs-seeded unseeded inserted debrided premolars root slices implanted subcutaneously immunodeficient mice. Samples retrieved after 30 days staining. independent samples t-test, analysis variance Kruskal–Wallis test used analyse quantitative data statistically depending on group numbers normality distribution. difference between groups considered significant when P-value less than 0.05. Results Acellular matrices generated following decellularization. Evaluation developed revealed reduction (P < 0.0001) preservation native histoarchitecture vasculature VEGF-A FGF-2. Upon recellularization hDPSCs, analyses cell engraftment progressive repopulation DDPs’ enhanced angiogenesis. In implantation apparent enhancement as compared DDP 0.0001). Conclusions had pro-angiogenic properties angiogenic factors. Seeding into led vasculature, improved capacity. se suggest that combination have potential provide promising promoting strategy regeneration.